Bachelor's degree in Biomedical Laboratory Techniques (to qualify as a biomedical laboratory worker) (Verona)

Diagnostic methodologies for Biochemistry and Molecular Biology (2019/2020)

Course code
4S000351
Credits
8
Coordinator
Martina Montagnana

Teaching is organised as follows:
Unit Credits Academic sector Period Academic staff
BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE 1 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY Lezioni TLB 1 SEMESTRE Mauro Negri
METODOLOGIA DIAGNOSTICA MOLECOLARE 2 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY Lezioni TLB 1 SEMESTRE Martina Montagnana
METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE 2 MED/46-BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE Lezioni TLB 1 SEMESTRE Andrea Mafficini
BIOTECNOLOGIE RICOMBINANTI 1 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY Lezioni TLB 1 SEMESTRE Elisa Danese
BIOLOGIA MOLECOLARE 2 BIO/11-MOLECULAR BIOLOGY Lezioni TLB 1 SEMESTRE Elisa Danese

Learning outcomes

MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
The course aims to acquaint the student of biochemistry and chemistry of environmental pollution: 1) specific terminology used 2) the mechanisms of pollution and depollution 3) the reasons for which man pollutes 4) the effects and the costs of pollution 5) the direct and indirect techniques of analysis of air, water and soil pollutants

MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide students who have no medical training the basic knowledge on the clinical aspects concerning the genetic basis of diseases. The course aims to provide an updated view of molecular biology techniques that are used in a clinical setting.

MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
The training objective is to provide adequate knowledge of the theoretical principles and application of the techniques for detection of genetic alterations, in a functional framework considering the main diseases related to alterations of genes, including cancer.

MM: BIOTECNOLOGIE RICOMBINANTI
The course aims to make students understand the basic stages of the birth of modern biotechnologies and the current applications of recombinant biotechnology in biomedical and pharmaceutical fields. We will discuss issues of a scientific, ethical and biological nature. The course also aims to provide an updated view of recombinant techniques including techniques for reproductive and therapeutic cloning, technic for the reproduction of recombinant drugs.

MM: BIOLOGIA MOLECOLARE
The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide an updated view of molecular biology techniques that are used in different clinical settings including prenatal diagnosis of genetic and chromosomal disorders, diagnosis of mendelian and oncoematologic diseases, infertility and pharmacogenetics.

Syllabus

MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE

MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
• Introduction and organization of the molecular biology laboratory • Extraction of nucleic acids • Electrophoresis of nucleic acids • Hybridization dot/blot and reverse dot/blot • Clinical molecular biology in hematological field • CBC and peripheral blood smear • Main methods of proteomics (chromatography, spettrometry..) • FISH and Array-CGH

MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE


MM: BIOTECNOLOGIE RICOMBINANTI
1) History of modern Biotecnology. The Reproductive cloning. The Therapeutic cloning. Critical review of two fundamental scientific works: 1) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH.Viable offspring derived from fetal and adult mammalian cells.Nature. 1997 Feb 27;385(6619):810-3. 2) Schwartz SD, Regillo CD, Lam BL, Eliott D, Rosenfeld PJ, Gregori NZ, Hubschman JP, Davis JL, Heilwell G, Spirn M, Maguire J, Gay R, Bateman J, Ostrick RM5, Morris D, Vincent M, Anglade E, Del Priore LV, Lanza R. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies. Lancet. 2015 Feb 7;385(9967):509-16. 2) Cloning vectors. Role of DNA ligase restriction enzymes, alkaline phosphatase. Plasmids. Methods for introducing DNA into a host cell: transformation and transfection. Vectors selection and isolation. 3) Transgenic animals. Gene transfer techniques: irestriction-enzyme-mediated integration (REMI), DNA microinjection, Sperm-mediated gene transfer, Retrovirus-mediated gene transfer. Gene targeting. knockout mice. 4) Clinical applications of recombinant drugs. Human recombinant TSH. production technique, commercial products, pharmacokinetic properties, approval for clinical use. Use in the diagnosis and follow-up of differentiated thyroid carcinoma 5) Clinical applications of recombinant drugs. Recombinant erythropoietin: production technique, commercial products, pharmacokinetic properties, approval for clinical use. Recombinant insulin: production technique, pharmacokinetic properties, clinical use, commercial products.

MM: BIOLOGIA MOLECOLARE
•Introduction and organization of the molecular biology laboratory •Cystic fibrosis: pathogenesis, clinical manifestations and diagnosis •Male infertility: laboratory tests in the diagnosis of congenital causes •Molecular biology in oncohematological diseases •Prenatal diagnosis: molecular and cytogenetic testing •Diagnostics of Genetic diseases in mental retardation •Hemocromatosis: pathogenesis, clinical manifestations and diagnosis •Application of Pharmaco-genetics and -genomics

Assessment methods and criteria

MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
Oral exam

MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
Written test, with open questions and multi choice questions. The exam is based on all the course contents. The exam is passed if the evaluation is greater or equal to 18/30.

MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
Oral Exam

MM: BIOTECNOLOGIE RICOMBINANTI
Oral exam

MM: BIOLOGIA MOLECOLARE
Oral exam

Reference books
Author Title Publisher Year ISBN Note
Ciaccio M, Lippi G Biochimica Clinica e Medicina di Laboratorio (Edizione 2017) EdiSES Edizioni Scientifiche ed Universitarie 2017 9788879599580
Neri G e Genuardi M Genetica Umana e Medica (Edizione 3) EDRA LSWR - Masson 2014 978-88-217-3743-4
APPUNTI DELLE LEZIONI Appunti delle lezioni forniti dal docente. 2019
Greg Gibson, Spencer V. Muse A primer of Genome Science (Edizione 3) Sinauer 2009 978-0-87893-236-8
Terry A. Brown Biotecnologie molecolari (Edizione 2) Zanichelli 2017 978-88-08-32096-4
Mauro Maccarrone Metodologie Biochimiche e biomolecolari (Edizione 1) Zanichelli 2019 978-88-08-52055-5
Lizabeth A. Allison Fundamental Molecular Biology Blackwell publishing 2002 978-1-4051-0379-4




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