Bachelor's degree in Biomedical Laboratory Techniques (to qualify as a biomedical laboratory worker) (Verona)

Diagnostic methodologies for Biochemistry and Molecular Biology (2015/2016)

Course code
4S000351
Credits
8
Coordinator
Maria Romanelli

Teaching is organised as follows:
Unit Credits Academic sector Period Academic staff
METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE 2 MED/46-BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE lez 3 anno 1 semestre TLB Marco Ligozzi
BIOTECNOLOGIE RICOMBINANTI 1 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY lez 3 anno 1 semestre TLB Maria Romanelli
BIOLOGIA MOLECOLARE 2 BIO/11-MOLECULAR BIOLOGY lez 3 anno 1 semestre TLB Elisa Danese
BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE 1 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY lez 3 anno 1 semestre TLB Mauro Negri
METODOLOGIA DIAGNOSTICA MOLECOLARE 2 BIO/12-CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY lez 3 anno 1 semestre TLB Martina Montagnana

Learning outcomes

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MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
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The training objective is to provide adequate knowledge of the main diseases related to alterations of genes and theoretical principles of the techniques of cytogenetics and molecular genetics. Obtain adequate knowledge of the theoretical principles and applications of the techniques of detection of genetic alterations.
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MM: BIOTECNOLOGIE RICOMBINANTI
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The course has the following objectives: to distinguish the activities of key enzymes used in recombinant technologies ; b ) provide the student with the knowledge necessary to understand the techniques applied for producing cloning vectors and gene expression vectors.
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MM: BIOLOGIA MOLECOLARE
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The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide an updated view of molecular biology techniques that are used in different clinical settings including prenatal diagnosis of genetic and chromosomal disorders, diagnosis of mendelian and oncoematologic diseases, infertility and pharmacogenetics.
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MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
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MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
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The course aims to make students understand the role of genetic testing in the clinical course of the patient. The course also aims to provide students who have no medical training the basic knowledge on the clinical aspects concerning the genetic basis of diseases. The course aims to provide an updated view of molecular biology techniques that are used in a clinical setting.

Syllabus

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MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
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Human genome organization, Genetic polymorphisms, Nucleic acid extraction, Nucleic acids labeling and quantification, Gene amplification techniques: Signal Amplification, Target amplification , Chromosomal aberrations, Conventional cytogenetics, Molecular cytogenetics, In situ hybridization FISH, Next generation Sequencing NGS, QF PCR, Array CGH, MLPA PCR, HLA typing, Genetic testing for clotting disorders, Tumor genetics, Forensic genetics. Pharmacogenetics, Genomic imprinting
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MM: BIOTECNOLOGIE RICOMBINANTI
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• Analysis of DNA by restriction and modification enzymes, restriction maps interpretation.. • Cloning and expressing vectors: the Luc and GFP reporter gene vectors. • Strategies for gene cloning and gene expression analysis. • Applications in the biomedical field of recombinant DNA technology. • Critical commentary of a protocol applied to epigenetics analysis.
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MM: BIOLOGIA MOLECOLARE
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•Introduction and organization of the molecular biology laboratory •Cystic fibrosis: pathogenesis, clinical manifestations and diagnosis •Male infertility: laboratory tests in the diagnosis of congenital causes •Molecular biology in oncohematological diseases •Prenatal diagnosis: molecular and cytogenetic testing •Diagnostics of Genetic diseases in mental retardation •Hemocromatosis: pathogenesis, clinical manifestations and diagnosis •Application of Pharmaco-genetics and -genomics
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MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
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MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
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• Introduction and organization of the molecular biology laboratory • Extraction of nucleic acids • Electrophoresis of nucleic acids • Hybridization dot/blot and reverse dot/blot • Microarrays • PCR e Real-time PCR • Sequencing and next generation sequencing • Main methods of proteomics (chromatography, spettrometry..) • FISH and Array-CGH

Assessment methods and criteria

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MM: METODI E TECNICHE DI GENETICA E BIOLOGIA MOLECOLARE
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Oral and/or written reports
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MM: BIOTECNOLOGIE RICOMBINANTI
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Written and oral examination
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MM: BIOLOGIA MOLECOLARE
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oral exam
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MM: BIOCHIMICA E CHIMICA DELL'INQUINAMENTO AMBIENTALE
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MM: METODOLOGIA DIAGNOSTICA MOLECOLARE
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Written test.

Reference books
Author Title Publisher Year ISBN Note
Reed et al. Metodologie di base per le scienze biomolecolari Zanichelli 2002
Neri G e Genuardi M Genetica Umana e Medica (Edizione 3) EDRA LSWR - Masson 2014 978-88-217-3743-4




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