High Content Analysis. Quantitative Tools for Cell-Based Research and Screening


March 7th, 2017 – h. 1.00 : 2.00 p.m.
Dr. Andrew Barlow
Thermo Fisher - Product Specialist

Aula C Lente Didattica
Policlinico B.go Roma
Piazzale L. Scuro, 10 - 37134 Verona 


Cellomics is the discipline of quantitative cell analysis using bioimaging methods and informatics with a workflow involving three major components: image acquisition, image analysis, and data visualization and management. Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. HCS typically employs fluorescence imaging of adherent cells or tissues samples in a high- throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. Multi-parameter image processing provides quantitative data from cell populations preserving spatial information.

Combined with perturbations such as RNA interference, small molecules, CRISPR/Cas9- mediated genome engineering and mutations, such screens are a powerful method for gaining systematic insights into biological processes such as Angiogenesis, Autophagy, Cell viability, Mitochondrial health, Protein synthesis and dynamic localization, Transcription factor activation, Apoptosis-caspase, Cell cycle and mitotic index, Cytoskeletal rearrangement, Neurite outgrowth, Stem cell differentiation (see http://www.thermofisher.com/it/en/home/life-science/cell-analysis/cellular-imaging/high- content-screening/hcs-applications.html).

For a list of recent publications: https://tools.thermofisher.com/content/sfs/brochures/high- content-screening-publications.pdf

Local organization and contact:
Dr.ssa Maria Teresa Valenti: mariateresa.valenti@univr.it
Prof. Massimo Delledonne: massimo.delledonne@univr.it

Evento a cura del Centro piattaforme Tecnologiche
 
Referente
Maria Teresa  Valenti

Data inizio
7 marzo 2017

Data fine
7 marzo 2017

Dipartimenti Afferenti
Biotecnologie  -  Diagnostica e Sanità Pubblica  -  Informatica  -  Medicina  -  Neuroscienze, Biomedicina e Movimento


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